Welcome to The Readout! Each quarter, we share a selection of highlights, updates, and resources for innovative investigators like you. Let’s jump right in — and happy reading.
Publication highlight
Atlas-guided discovery of transcription factors for T cell programming
Chung, H.K., Liu, C., Battu, A. et al. Atlas-guided discovery of transcription factors for T cell programming. Nature (2026). https://doi.org/10.1038/s41586-025-09989-7
A new regulatory atlas maps the transcription factors (TFs) governing CD8+ T cell differentiation, identifying ZSCAN20 and JDP2 as primary drivers of exhaustion. By leveraging 10x Genomics Chromium 5’ assays for single cell CRISPR screens, researchers demonstrated that precision TF deletion can prevent cell fatigue while maintaining protective immunity, offering a new blueprint for resilient CAR-T therapies.
Key findings
Targeting exhaustion: Identifying specific "molecular switches" to prevent T cell failure.
High-throughput mapping: Using single cell data to catalog fingerprints across nine distinct cell states.
Therapeutic engineering: Providing a roadmap for more durable cancer immunotherapies.
Got inspiration for your own single cell CRISPR screening experiment? See our resources below for more food for thought and expert tips on getting started:
Apex reflects the latest advancement within the Flex product family, offering high sensitivity, scalable performance, and broad sample compatibility, including FFPE, along with streamlined sample handling.
Please note that while the product name has changed, kit components and technical documentation will continue to reference GEM-X Flex v2 during the transition period.
Download the updated Flex Apex product sheet and Chromium X Series assay compatibility brochure.
Our spatial analysis portfolio is now expanded with Xenium Protein subpanels — enabling targeted gene + protein expression in situ, ideal for focused spatial discovery experiments. This spatial capability helps researchers combine cell location and proteomic phenotypes in a single assay.
Planning your next experiment? Check out our Experiment Builder, now available for all 10x platforms. Just specify your conditions, and the tool instantly identifies the products you’ll need along with an estimated project cost. It’s a simple way to align experimental design with budget planning — all in one place.
Analysis of 10x Genomics data is full of possibilities. Here is a collection of introductions, tutorials, and blogs for data analysis beyond software developed by 10x Genomics. We hope these guides help you navigate your exciting analysis journey.
Looking for a best fit for your experiment? See comparison between our products here. And visit our regularly updated Knowledge Base Q&A for more expert tips.
Stay in the loop! Did you know we have a newsletter just for technology updates? Sign up and choose Product Updates, Technical Workflow Updates, and Technical Software Updates to get the latest news delivered straight to your inbox.
Fueling deeper discovery: meet our new partners
In this edition, we are pleased to announce four new partnerships aimed to broaden the reach of our technologies. These collaborations represent an important step forward, creating new avenues for innovation and data-driven discovery.
We are excited to share that PharosAI, a UK-based research consortium, has selected our Xenium spatial platform to help build one of the world’s most comprehensive multimodal cancer datasets. This consortium brings together King's College London, Queen Mary University of London, Guy's and St Thomas' NHS Foundation Trust and Barts Health NHS Trust to transform decades of archived NHS cancer samples into high-resolution, AI-ready datasets.
PharosAI will democratize access to these datasets, making them securely available to a broad community of researchers and innovators. Read the full announcement.
Our new collaboration with BioTuring aims to address the challenge many researchers face with large multimodal datasets, where analysis — rather than data generation — is the primary bottleneck.This partnership connects our technologies with BioTuring’s advanced analytical ecosystem to better link data generation with downstream analysis. Read the full announcement.
Cancer Research Institute Launches Transformative AI-Driven Immuno-Oncology Initiative Powered by 10x Genomics Technology
Multi-phase initiative will generate single cell and spatial data from over 20,000 samples across leading immuno-oncology laboratories to map the immune landscape of cancer and drive the next generation of immunotherapies and vaccine discovery. Read the full announcement
CareDxand 10x Genomics to Launch ImmuneScape™ Program – A Multiomics Research Platform to Decode Transplant Rejection and Drug Response
Collaboration will engage leading transplant clinician-scientists to study antibody-mediated rejection (AMR) and microvascular inflammation (MVI) using cellular-resolution profiling. Read the full announcement
Where to meet us
In-person
High-Content CRISPR Screening Conference, March 18-19, Vienna, Austria. Hear from Peter Smibert, our Vice President of Biology during CRISPR Screening with Single-Cell Readout session on March 18.
Association for Cancer Immunotherapy CIMT Annual Meeting, May 11-13, Mainz, Germany.
Every sample has a story. It could change someone’s life.
You are invited to our Single Cell and Spatial Biomarker Discovery Summit. This online event brings together top translational researchers to explore the future of precision medicine. Discover cutting-edge techniques and dive deep into the future of research during our dedicated panel discussion on generating and leveraging AI-grade data.
Featured speakers and panelists: Almudena Espin Perez, OWKIN; Raphael Gottardo, University of Lausanne; Eric Letouzé, One Biosciences; Florian Rambow, University Clinic Essen; Omer Bayraktar, Wellcome Sanger Institute; Ronny Baber, University Hospital Leipzig; Olaf Heidenreich, Princess Máxima Center for Pediatric Oncology; Martin Seifert, 10x Genomics; panel discussion is moderated by Nigel Delaney, Vice President, Computational Biology, 10x Genomics.
…curiosity and technology together move us forward. That is the key to innovation. – Martin Seifert, Sr. Science & Technology Advisor, 10x Genomics
This conversation between Dr. Martin Seifert, and Prof. Thomas Bosch was recorded during the 2025 KLS Summer School “Spatial Biology in Health and Disease” at Kiel University.
As there is more capacity to generate single cell and spatial data, Martin and Thomas highlight the importance of bioinformatics and how collaboration with university life science hubs moves forward developments of new algorithms and enables new ways to interpret the data.
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Did you know that we offer grant application support?
We’re here to help! Check out our Grant Assistant. If you need a Letter of Support, we typically process requests within one business day — so you can hit your deadline with time to spare.
Uncover molecular insights, dissect cell-type differences, detect novel subtypes and biomarkers, decipher spatial gene expression patterns, and more... Get in touch to explore the possibilities for your research.
