Date and Location:
June 30, 2022
9:00 AM - 5:00 PM CT
Greehey Children’s Cancer Research Institute
8403 Floyd Curl
San Antonio, TX 78229
Please join us to learn how single cell, spatial, and in situ technologies from 10x Genomics can help you push the boundaries of your research. Uncover molecular insights, dissect cell-type differences, investigate the adaptive immune system, detect novel subtypes and biomarkers, and map the epigenetic landscape cell by cell. Enabling deeper insight into cancer, immunology, neuroscience, and developmental biology, 10x Genomics gives researchers the ability to see biology in new ways.
We'll start with a technology overview of our single cell and spatial genomics solutions followed by practical presentation on how to prepare and analyze your samples. The day will conclude with time to meet one on one with a 10x-Pert to discuss your project ideas.
Agenda
10:00 AM - 10:30 AM - Gain a multidimensional view of biology with single cell solutions
Spence Fast, MS., Science and Technology Advisor, 10x Genomics
See how 10x Genomics is helping researchers uncover molecular insights, explore cellular heterogeneity, dissect cell-type differences, investigate the innate and adaptive immune system, identify rare cell types, detect novel biomarkers, and map the epigenetic landscape cell by cell with Chromium Single Cell solutions
10:30 AM - 11:00 AM - Spatially map and quantify gene expression in the tissue context with Visium Spatial
Leilani Marty Santos, PhD., Spatial Science and Technology Advisor, 10x Genomics
Map the whole transcriptome of FFPE or fresh frozen tissue sections with morphological context to discover novel insights into normal development, cell atlasing, disease pathology, and clinical translational research.
11:00 AM - 11:30 AM - Chromium Single Cell Fixed RNA Profiling
Jason Koth, Product Manager - Single Cell, 10x Genomics
Enable storage for transport and/or batched runs in single cell experiments with PFA fixation, without compromising sample integrity or data quality. Streamline single cell workflows, improve sample accessibility, and overcome logistical challenges by preserving fragile biology at the point of collection.
11:30 AM - 12:30 PM - Lunch
12:30 PM - 1:00 PM - Single Cell Gene Expression: Practical considerations and best practices for sample preparation
Fatjon Leti, Field Applications Scientist Single Cell Specialist, 10x Genomics
Learn how to get the most out of 10x Genomics Single Cell Gene Expression. Go beyond traditional bulk gene expression analysis to reveal the true complexity of cellular diversity. Starting with an overview of the workflow, this seminar provides key considerations for experimental design, and best practice guidance for successful sample preparation when you perform single cell gene expression analysis.
1:00 PM - 1:30 PM - Tips and Tricks: Sample preparation for Visium assays
Mahdis Rhamani, Field Applications Scientist Tissue Specialist, 10x Genomics
Join us to learn about critical sample prep decisions and technical considerations for sample preparation of fresh frozen and FFPE samples including tissue sectioning, slide preparation, H&E staining, IF staining, microscopy, imaging, and library construction.
1:30 PM - 2:00 PM - Analyzing spatial and high-resolution single cell multiomic data
Fatjon Leti, Field Applications Scientist Single Cell Specialist, 10x Genomics
To enable the interpretation of complex single cell and spatial transcriptomic data sets, 10x Genomics provides analysis and visualization software for evaluating genomic, epigenomic, transcriptomic, and proteomic data. Join us as we discuss software, methods, and guidance that can be used to analyze your 10x Genomics datasets.
2:00 PM - 3:30 PM - Meet your local 10x Team
