Human induced pluripotent stem cells (hiPSCs) offer powerful models for human development and disease. Implementing scalable and temporally controlled CRISPR regulation in these cells remains challenging.
In this webinar, we present combinatorial CRISPR perturbations in human iPSCs, resolved at single cell resolution, to engineer and dissect cell-fate transitions and map the transcription factor combinations driving differentiation.
From single to combinatorial CRISPRa screens: Mapping transcription factor-driven differentiation in iPSCs, Sejla Salic-Hainzl, bit.bio
Sejla shares how pooled single cell CRISPRa screens in human iPSCs systematically profile transcription factors driving differentiation, and how single and combinatorial perturbations shape emerging cell identities. She discusses why sensitive sgRNA detection is essential for combinatorial screens, and how these datasets support predictive modeling for future cell-type discovery.
Programming human iPSC fate with combinatorial, inducible CRISPRa/i and single cell genomics, Alessandro Bertero, University of Torino
Alessandro introduces CIRI, an isogenic platform for combinatorial inducible CRISPR in hiPSCs enabling coordinated activation and repression of endogenous genes from a shared dCas9 chassis. He shows how simultaneous MYOD1 activation and pluripotency-factor repression improves myogenic forward programming, and how single cell CRISPR screens uncover regulatory combinations that refine differentiation outcomes.
Discover how Flex Apex and the new VIPerturb-seq method make genome-wide, single-cell CRISPR perturbation screens more sensitive, scalable, and affordable — enabling up to a million perturbations with just ~2,000 probes, Stacey Abidayo, 10x Genomics
VP of Research and Development
bit.bio
Associate Professor in the Department of Molecular Biotechnology and Health Sciences
University of Torino
Staff Product Manager, Single Cell
10x Genomics